Composite

Part:BBa_K2922035:Design

Designed by: Yunyue Shi   Group: iGEM19_XMU-China   (2019-10-13)


The colicin-N operon under pBAD(Arabinose promoter) control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 696
    Illegal AgeI site found at 1700
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Each CDS are added an RBS (BBa_B0034) behind, and contain a terminator(BBa_B0010)


Source

This part constructs from BBa_K206000,BBa_K206000,BBa_K2922028,BBa_K2922029 by standard assembly.

References